# how do you calculate magnification on a microscope

how do you calculate magnification on a microscope Way1

Step1 Locate the power of the eye piece, which is usually 10X. It is typically written on the top of the microscope on the eyepiece.

Step2-Determine the power of the objective that you are using to examine the slide. Typically viewing powers are 4X, 10X and 40X.

Step3-Multiply the viewing power of the eye piece by the power of the objective used to examine the slide. For example, if the viewing power is 10X and you use the power of the objective at 10X, then the magnification is 100X.

how do you calculate magnification on a microscope Way2

Compound light microscopes use a series of lenses and visible light to magnify objects. The magnification allows the user to view bacteria, individual cells and some cell components. In order to calculate the magnification, the power of the ocular and objective lenses is needed. The ocular lens is located in the eye piece. The scope also has one to four objective lenses located on a rotating wheel above the platform. The total magnification is the product of the ocular and objective lenses.

Calculate Magnification of a Compound Light Microscope

Determine the magnification strength of the ocular lens. This should be written on the outside of the eye piece, but if it is not look in the manual. Generally speaking the ocular lens magnifies 10x.

Determine the magnification capacity of the objective lens. The magnification is written on the side of the lens. Traditionally, the value could be 4x, 10x, 40x, or 100x. If you are not sure of the magnification power, check the manual. The objective lens is located on the rotating wheel just above the stage or platform where you place the microscope slide. In some instances the microscope may have only one lens, but generally it has three to four.

To calculate the total magnification of the compound light microscope multiply the magnification power of the ocular lens by the power of the objective lens. For instance, a 10x ocular and a 40x objective would have a 400x total magnification. The highest total magnification for a compound light microscope is 1000x.

how do you calculate magnification on a microscope Way13

The magnification of a microscope describes the increase in apparent size of an object compared with its actual size. An object magnified 10 times (10X) appears 10 times larger than it really is. Total magnification is the product of the ocular lens magnification and the objective lens magnification. Magnification does not describe the quality of the image. Magnifying an object without good resolution is called empty magnification, as the image appears larger but no greater detail can be seen. Resolution typically limits the usefulness of light microscopes rather than magnification.

Record the magnification of the ocular lens in the eyepiece. The magnification of the ocular lens is typically engraved on the side of the eyepiece.

Record the magnification of the objective lens. The magnification is frequently engraved along with the numerical aperture (NA) on the side of the objective lens. Many compound light microscopes allow different objective lenses to be rotated into use on the nosepiece. Each objective lens has a different magnification.

Multiply the magnification of the eyepiece by the magnification of the objective lens to produce total magnification. For example, a 10X ocular lens and a 40X objective lens will produce a total magnification of 400X (10 x 40 = 400). Changing the ocular lens or objective lens to lenses with a different magnification will change the total magnification of the microscope. Usually, it is the objective lens that is changed to increase or decrease magnification.

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