how to determine the magnification of a microscope
how to determine the magnification of a microscope
how to determine the magnification of a microscope
How to Calculate Magnification of a Microscope
If you have ever been in a biology lab, then you have probably used a microscope to view slides. Microscopes help us magnify tiny objects so that you can view them more easily. This is important when you want to view an object on the cellular level. They come equipped with adjustable settings so that you can view the slides at different powers.
By AlysonNolan
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If you have ever been in a biology lab, then you have probably used a microscope to view slides. Microscopes help us magnify tiny objects so that you can view them more easily. This is important when you want to view an object on the cellular level. They come equipped with adjustable settings so that you can view the slides at different powers.
…
Dials on the side of the microscope adjust the magnification level.
Video of the Day
Step 1
Locate the power of the eye piece, which is usually 10X. It is typically written on the top of the microscope on the eyepiece.
Step 2
Determine the power of the objective that you are using to examine the slide. Typically viewing powers are 4X, 10X and 40X.
Step 3
Multiply the viewing power of the eye piece by the power of the objective used to examine the slide. For example, if the viewing power is 10X and you use the power of the objective at 10X, then the magnification is 100X.
how to determine the magnification of a microscope
How To Calculate Total Magnification
This article describes how to calculate the effective magnification and the size of your sample on your monitor when using a digital camera on a microscope.
Magnification on the TV
Monitor
In order to determine the total
magnification on the TV monitor, the following variables must be known:
Objective magnification
Microscope adapter magnification.
CCD chip diagonal dimension
TV monitor diagonal dimension
The formula used to calculate total magnification on the TV monitor is:
Total Magnification = Objective Magnification x Microscope Adapter Magnification x Video Magnification
The video magnification is determined by dividing the TV monitor diagonal (mm) by the CCD chip diagonal (mm). A reference chart is given below which shows video magnification for various monitor and chip sizes. To determine “Video Magnification” on a computer monitor divide the actual
image diagonal (as measured on the screen) by the CCD chip diagonal.
how to determine the magnification of a microscope
diagonal.

9″ 
12″ 
13″ 
19″ 
20″ 
27″ 
1/3″ 
38.1x 
50.8x 
55.0x 
80.4x 
84.7x 
114.3x 
1/2″ 
28.6x 
38.1x 
41.3x 
60.3x 
63.5x 
85.7x 
2/3″ 
20.8x 
27.7x 
30.0x 
43.9x 
46.2x 
62.3x 
1.0″ 
14.3x 
19.1x 
20.6x 
30.2x 
31.8x 
42.9x 
What is the magnification on the TV monitor when using a 10X objective, 0.45x video coupler, a 1/2″ format CCD and a 19″ monitor?

482.6 mm (19″
diagonal)
——————————
8 mm chip diagonal 
Total
Magnification 
= 
10 
x 
0.45 
x 
= 
10 
x 
0.45 
x 
60.3 
= 
271.35 
Determining
the Size of Your Specimen
The approximate real size of a specimen can be determined by dividing the length of the specimen measured on the monitor screen by the total magnification on the monitor.

Length of Specimen Measured on Monitor Screen
———————————————————————
Total Magnification 
Real
Size of Specimen 
= 


In the above example, if the measured length of a specimen was 100 mm, the
real size of the specimen would be:
Real
Size of Specimen 
= 
100 mm
/ 271.35 
= 
0.37 mm 
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