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how to use a compound light microscope step by step

how to use a compound light microscope step by step

  1. Turn the revolving turret (2) so that the lowest power objective lens (eg. 4x) is clicked into position.
  2. Place the microscope slide on the stage (6) and fasten it with the stage clips.
  3. Look at the objective lens (3) and the stage from the side and turn the focus knob (4) so the stage moves upward. Move it up as far as it will go without letting the objective touch the coverslip.
  4. Look through the eyepiece (1) and move the focus knob until the image comes into focus.
  5. Adjust the condenser (7) and light intensity for the greatest amount of light.
  6. Move the microscope slide around until the sample is in the centre of the field of view (what you see).
  7. Use the focus knob (4) to place the sample into focus and readjust the condenser (7) and light intensity for the clearest image (with low power objectives you might need to reduce the light intensity or shut the condenser).
  8. When you have a clear image of your sample with the lowest power objective, you can change to the next objective lenses. You might need to readjust the sample into focus and/or readjust the condenser and light intensity. If you cannot focus on your specimen, repeat steps 3 through 5 with the higher power objective lens in place. Do not let the objective lens touch the slide!
  9. When finished, lower the stage, click the low power lens into position and remove the slide.

How to Adjust compound light microscope?

Some compound microscopes come with what is called a rack stop. A rack stop prevents the objective lenses from being lowered into the slide.

However, some older microscopes do not have a rack stop, so it is always advisable to check, just in case. Lowering an objective lens into a slide could easily break the slide and damage the sample.

In order to move your microscope safely, one hand should be under its base for support and the other at its arm. Be sure to only switch off the microscope when the dimmer is set to the lowest intensity and always turn off the lamp before moving the microscope.

So now you know how to use and adjust a compound microscope. Well done. Happy Viewing!

10. Now adjust the small fine focus knob until the specimen is clearly in focus. Then adjust the diaphragm to get the best lighting. Start with the most light and gradually lessen it until the specimen image has clear, sharp contrast.

11. Once you have attained a clear image, scan the slide (right to left and top to bottom) at low power to get an overview of the specimen.Then center the part of the specimen you want to view at higher power. You should be able to change to a higher power objective lens with only minimal use of the focusing adjustment. If you cannot focus on your specimen, repeat the above steps and work from objective to objective until the higher power objective lens is in place.

12. Rotate the nosepiece to the 10x objective for 100x magnification. Refocus and view your specimen carefully. Adjust the lighting again until the image is most clear (you will need more light for higher power).

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